Recombinant Human IL22 Protein, His-tagged

• Cat.No.: IL22-217H
• Product Overview: Biotinylated Recombinant Human Interleukin-22 is produced by Human 293 Cells. The target gene encoding A34-I179 is expressed with a 6His tag at the C terminus.

Specification

• Description: Interleukin-22 (IL22) is a cytokine that belongs to the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 initiated its biological activity by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S1s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized frum a 0.2 um filtered solution of PBS, pH7.2.
• Molecular Mass: Predicted Molecular Weight: 20.8kDa
  Apparent Molecular Weight: Biotinylated IL-22 protein appeared as a smear between 25-40kDa in a reducing SDS-PAGE gel due to glycosylation.
• AA Sequence: A34-I179
• Endotoxin: Less than 0.1 EU/ug.
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE
• Shipping: The product is shipped on dry ice pack.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: IL22 interleukin 22 [ Homo sapiens (human) ]
• Official Symbol: IL22
• Synonyms: Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL22; ZCYTO18; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
• Gene ID: 50616
• mRNA Refseq: NM_020525.5
• Protein Refseq: NP_065386.1
• MIM: 605330
• UniProt ID: Q9GZX6

Reviews

04/07/2021

High specificity.

03/29/2021

Good for Th17 differentiation.

07/07/2016

Effective in gut repair assays.

Q&As

What is IL22's role in gut epithelial repair?

IL22 enhances gut epithelial repair mechanisms.

What are the downstream targets of IL22 signaling?

IL22 activates STAT3 to modulate downstream targets.

How does IL22 interact with other cytokines in inflammation?

IL22 interacts synergistically with IL-17 during inflammation.

Is IL22 involved in the pathogenesis of autoimmune diseases?

IL22 is involved in the pathogenesis of autoimmune diseases like Crohn's.

Does IL22 have a role in metabolic syndrome?

IL22 is implicated in metabolic syndrome by affecting insulin sensitivity.

How is IL22 expression regulated in psoriasis?

IL22 expression is upregulated in psoriasis.

Does IL22 expression change during viral infections?

IL22 expression is upregulated during viral infections like Hepatitis C.

Does IL22 contribute to anti-tumor immunity?

IL22 contributes to anti-tumor immunity by activating NK cells.

How does IL22 modulate T-cell differentiation?

IL22 promotes Th22 cell differentiation.

What impact do IL22 polymorphisms have on its function?

IL22 polymorphisms affect its binding affinity and signaling.