Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged IL23A&IL12B-335HM

Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged

Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged Online Inquiry

IL23A&IL12B Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : IL23A&IL12B-335HM Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # NP_057668 (IL23A) & NP_032378 (IL12B)). Predicted N-terminus: His (IL23A) & Met 23 (IL12B). Human IL-23 alpha & Mouse IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Met 23 (IL12B). The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 45-50 kDa (IL-12 beta) respectively due to glycosylation.
Source : HEK293
Species : Human & Mouse
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged at 10 μg/mL (100 μL/well) can bind Human IL23R with a linear range of 0.039-0.625 μg/mL (Routinely tested).
Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged stimulates secretion of IL-17 by mouse spleen cells. The ED50 for this effect is 0.0069-0.0426 ng/ml (Routinely tested).
Cell based assay shows that the secretion of IL-17 induced by Recombinant Human IL23A & Mouse IL12B Heterodimer Protein, His-tagged is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.41-1.012 ng/mL (Routinely tested).
Molecular Mass : 19.5 kDa (IL23A) & 35.8 kDa (IL12B)
Protein length : Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B)
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ] & Il12b interleukin 12b [ Mus musculus ]
Official Symbol : IL23A & IL12B
Synonyms : IL23A; IL 23; IL 23A; IL23P19; P19; SGRF; IL-23-A; IL-23p19; IL-23; IL-23A; MGC79388; & IL12B; interleukin 12b; CLMF p40; IL-12 p40; p40; Il-12b; Il12p40; Il-12p40;
Gene ID : 51561 & 16160
mRNA Refseq : NM_016584 & NM_008352
Protein Refseq : NP_057668 & NP_032378
MIM : 605580
UniProt ID : Q9NPF7
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (0)

Ask a Question for All BRAF Products

Required fields are marked with *

  • Verification Code
×

Online Inquiry


  • Note: There will be extra charge for optional service!

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2023 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy