Active Recombinant Human IL3 Protein (Ala20-Phe152), GMP Grade, Animal-Free

• Cat.No.: IL3-26HG
• Product Overview: GMP recombinant human IL-3 protein (Ala20-Phe152) with and without an N-terminal Met was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Description: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.02-0.1 ng/mL.
  The specific activity of recombinant human IL-3 is >1.7 x 106 IU/mg, which is calibrated against the human IL-3 WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: 15 kDa
• Protein Length: Ala20-Phe152
• N-terminal Sequence Analysis: Met-Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu
  Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu-Lys
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700