IL32-00H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human IL32 (Met1-Lys188)Protein with a His tag at N-terminus was expressed in E. coli.
Description :
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Source :
E. coli
Species :
Human
Tag :
His
Bio-activity :
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells under serum free conditions in the presence of 10 μg/mL of Polymyxin B. Kim, S.H. et al. (2005) Immunity 22:131. The ED50 for this effect is 10-50 ng/mL.
Molecular Mass :
23.1 kDa
Protein length :
1-188
N-terminal Sequence Analysis :
Met
Endotoxin :
<1.0 EU/μg of the protein by the LAL method.
Purity :
>90% by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after opening. 3 months, -20 to -70 centigrade under sterile conditions after opening.
Storage Buffer :
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and CHAPS.
Shipping :
The product is shipped with dry ice or equivalent.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.