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Recombinant Human IL36B protein

Cat.No. : IL36B-509H
Product Overview : Recombinant Human IL36B protein (Interleukin-36 beta, 153a.a.) was expressed in Escherichia coli.
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Description : Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36 beta is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin and it can stimulate production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. It has two isoforms. IL-36β isoform 2 contains one potential N-linked glycosylation site in its C-terminus, while IL-36β isoform 1 lacks potential N-linked glycosylation sites and four of the conserved β-strands. Human IL-36βisoform 2 shares 62 %, 67 %, 63 % and 59 % a.a. identity with the most similar isoform of mouse, canine, bovine and equine IL-36β, respectively.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion in human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 10⁵ IU/mg.
Molecular Mass : Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.
Protein length : 153
AA Sequence : REAAPKSYAIRDSRQMVWVLSGNSL IAAPLSRSIKPVTLHLIACRDTEFS DKEKGNMVYLGIKGKDLCLFCAEIQ GKPTLQLKEKNIMDLYVEKKAQKPF LFFHNKEGSTSVFQSVSYPGWFIAT STTSGQPIFLTKERGITNNTNFYLD SVE
Endotoxin : Less than 1 EU/μg of rHuIL-36β, 153a.a. as determined by LAL method.
Purity : >97% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IL36B
Official Symbol : IL36B
Synonyms : IL36B; interleukin 36, beta; IL1F8, interleukin 1 family, member 8 (eta); interleukin-36 beta; FIL1; FILI (ETA); IL 1F8; IL 1H2; IL1 ETA; IL1H2; MGC126880; MGC126882; FIL1 eta; IL-1 eta; IL-1F8 (FIL1-eta); interleukin-1 eta; interleukin 1, eta; interleukin-1 homolog 2; Interleukin-1 Superfamily e; family of interleukin 1-eta; interleukin-1 family member 8; IL1F8 (Canonical product IL-1F8a); interleukin 1 family, member 8 (eta); FIL1H; IL1F8; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA);
Gene ID : 27177
mRNA Refseq : NM_014438
Protein Refseq : NP_055253
MIM : 605508
UniProt ID : Q9NZH7-2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the research topics related to IL36B? 06/28/2022

Research topics related to IL36B include in-depth study of its signal transduction mechanism, evaluation of therapeutic efficacy, and comparison with other therapeutic approaches.

What is the action mechanism of IL36B? 04/08/2022

IL36B exerts its therapeutic role by binding to its receptors, activating signaling pathways and regulating inflammatory and immune responses.

How long does the effect of IL36B last? 09/07/2021

The duration of the IL36B's efficacy will vary from individual to individual, and regular treatment is usually required to maintain the effect.

Are there other treatments similar to IL36B? 09/17/2020

Yes, there are other treatments similar to IL36B, such as antibody drugs and small molecule compounds.

How to determine the dosage and frequency of IL36B? 12/03/2019

The dosage and frequency of use are determined according to the specific circumstances of the patient and the results of the clinical trial, and are usually adjusted on an individual basis based on the clinical trial.

How was the IL36B developed and used in production? 05/16/2019

The development process of IL36B includes basic research, drug screening, clinical trials and other stages, and has been developed after long-term efforts

Customer Reviews (3)

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Reviews
10/11/2022

    When conducting experiments with IL36B, it is easy to operate and does not require complex operating steps.

    03/09/2022

      The stability of IL36B has good adaptability under different temperature and pH conditions.

      10/16/2021

        The structure of IL36B was validated with no abnormal protein folding or inactivation.

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