Description : |
Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36γ is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36γ also can function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Human IL-36γ (152a.a) shares 58 %, 59 %, 68 % and 69 % a.a. sequence identity with mouse, rat, bovine and equine IL-36γ, respectively, and 23 - 57% a.a. sequence identity with other family members. |
Source : |
E.coli |
Species : |
Human |
Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1 × 10⁵ IU/mg. |
Molecular Mass : |
Approximately 17.0 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
Protein length : |
152 |
AA Sequence : |
SMCKPITGTINDLNQQVWTLQGQNL VAVPRSDSVTPVTVAVITCKYPEAL EQGRGDPIYLGIQNPEMCLYCEKVG EQPTLQLKEQKIMDLYGQPEPVKPF LFYRAKTGRTSTLESVAFPDWFIAS SKRDQPIILTSELGKSYNTAFELNI ND |
Endotoxin : |
Less than 1 EU/μg of rHuIL-36γ, 152a.a. as determined by LAL method. |
Purity : |
>95% by SDS-PAGE and HPLC analysis. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20centigrade. Further dilutions should be made in appropriate buffered solutions. |