Active Recombinant Human IL4 Protein, His-Avi-tagged, Biotinylated
Cat.No. : | IL4-139H |
Product Overview : | Biotinylated Human IL-4 is expressed from HEK293 cells. It contains AA His 25 - Ser 153 (Accession # AAH67514). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Predicted N Terminal : | His 25 |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Human IL-4 R alpha, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human IL-4, His Tag with a linear range of 1.95-15.6 ug/mL. Biotinylated Human IL-4, His Tag, Avi Tag stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 1.19-2.64 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 17.6 kDa. As a result of glycosylation, the protein migrates as 22 kDa under reducing condition, and 21 kDa under non-reducing condition (SDS-PAGE). |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | IL4 interleukin 4 [ Homo sapiens ] |
Official Symbol : | IL4 |
Synonyms : | IL4; interleukin 4; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; BCGF 1; BCGF1; BSF1; IL 4; lymphocyte stimulatory factor 1; MGC79402; binetrakin; pitrakinra; IL-4; BSF-1; BCGF-1; |
Gene ID : | 3565 |
mRNA Refseq : | NM_000589 |
Protein Refseq : | NP_000580 |
MIM : | 147780 |
UniProt ID : | P05112 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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