Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human IL4 protein, His-tagged

Cat.No. : IL4-483H
  • Specification
  • Gene Information
  • Related Products
Product overview : Recombinant Human IL4 (P05112) aa. (His25~Ser153) fused with His tag was produced in HEK293F cells.
Description : The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Source : HEK293F
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 16.6kDa; Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
Protein length : His25~Ser153
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : > 95%
Characteristic : Isoelectric point is 9.3.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200ug/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Gene Name : IL4 interleukin 4 [ Homo sapiens (human) ]
Official Symbol : IL4
Synonyms : IL4; interleukin 4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1; interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
Gene ID : 3565
mRNA Refseq : NM_000589.3
Protein Refseq : NP_000580.1
UniProt ID : P05112

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (3)

Write a review
Reviews
10/17/2021

    By testing immunogenicity in animal models, detect whether an immune response is induced.

    11/09/2020

      The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

      05/06/2020

        This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.

        Ask a Question for All IL4 Products

        Required fields are marked with *

        My Review for All IL4 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends