Recombinant Human IL5

• Cat.No.: IL5-214H
• Product Overview: Recombinant Human interleukin 5 (colony-stimulating factor, eosinophil) DNA sequence encoding the human IL-5 protein sequence containing two 115 amino acid peptide chains was inserted into a suitable expression vector and was expressed inSf21 insect cells.

Specification

• Cat. No.: IL5-214H
• Description: Interleukin 5 or IL-5 is an interleukin produced by T helper-2 cells and mast cells. Its functions are to stimulate B cell growth and increase immunoglobulin secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a TH2 cytokine which is part of the hematopoietic family. Unlike other members of this cytokine family in its active form, this glycoprotein is a homodimer. IL-5 expression is regulated by several transcription factors including GATA-3.
• Source: Sf21 Insect Cells.
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-5 is Mr 32-34 kDa.
• State Of Matter: Solution.
• Formulation: This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human IL-5 was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1. The expected ED50 for this effect is typically 0.1 - 0.2 ng/mL.
• Storage And Stability: Working aliquots of this recombinant protein solution are stable for up to twelve months at -20˚C to -70˚C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2˚C - 8˚C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20˚C to -70˚C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); EDF; IL-5; TRF; B cell differentiation factor I; T-cell replacing factor; eosinophil differentiation factor; interleukin 5; interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; OTTHUMP00000065830
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• UniProt ID: P05113
• Chromosome Location: 5q31.1
• MIM: 147850
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Hematopoietic cell lineage; Allograft rejection; Asthma; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; T cell receptor signaling pathway
• Function: interleukin-5 receptor activity; cytokine activity; growth factor activity