Active Recombinant Human IL5 protein, His&hFc-tagged

• Cat.No.: IL5-185H
• Product Overview: Active Recombinant Human IL5 protein(P05113)(20~134 aa), fused with N-terminal His tag and C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 20-134 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Interleukin-5 (IL-5), a secreted glycoprotein, is a member of the hematopoietic family. Interleukin 5 has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 2 x 10^4 cells/well and incubated for 72h in the presence or absence of various concentrations of recombinant human IL-5 at 37°C. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37°C . Cell proliferation of TF-1 cells after incubation with IL-5 for 72h observed by inverted microscope was shown. The dose-effect curve of recombinant human IL-5 was shown. It was obvious that it significantly promoted cell proliferation of TF-1 cells. The ED50 for this effect is typically 35 ng/ml.
• Molecular Mass: 44.2kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
• Official Symbol: IL5
• Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5
• Gene ID: 3567
• mRNA Refseq: NM_000879
• Protein Refseq: NP_000870
• MIM: 147850
• UniProt ID: P05113