| Cat.No. : |
IL6RA-345H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human IL6RA protein (Leu20-Pro365), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293). |
| Description : |
Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126), is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
human IgG1 Fc |
| Form : |
Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 65.0 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
346 |
| Endotoxin : |
Less than 1.0 EU per ug by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of IL6RA-345H : |
Human IL6R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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| Bioactivity-ELISA of IL6RA-345H : |
Immobilized Human IL-6, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.