| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
26-177 aa |
| Form : |
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA.Immobilized Human IL7 at 2 μg/mL (100 μL/well) can bind human IL7R with a linear range of 0.97 -159.1 ng/mL. 2. Measured in a cell proliferation assay using IL-2-activated mouse spleen cell. The ED50 for this effect is 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×10^6~1×10^7 units/mg. 3. Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.78-3.1ng/mL, corresponding to a specific activity of 3.23×10^5~1.28×10^6 units/mg. |
| Molecular Mass : |
18.23 kDa |
| Endotoxin : |
< 0.1 EU/μg of the protein by LAL method. |
| Purity : |
≥ 90 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC. |
| Storage : |
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| Reconstitution : |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
