Recombinant Human IL7RA protein, Fc-tagged
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|Cat.No. :||IL7RA-328H Optional Service: Optional requirements on this protein|
|Product Overview :||Recombinant Human IL7RA protein (Glu21-Gly236), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra/CD127 contains 1 fibronectin type-III domain. IL7Ra/CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra/CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.|
|Tag :||human IgG1 Fc|
|Form :||Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 51.0 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||216|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IL7RA-328H :||
Human IL-7 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of IL7RA-328H :||
Immobilized Human IL-7, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Fc Tag with a linear range of 1-16 ng/mL (QC tested).
|Bioactivity-SPR of IL7RA-328H :||
Captured Human IL-7R alpha, Fc Tag on CM5 chip via anti-human IgG Fc antibodies surface can bind ActiveMax® Human IL-7, Tag Free with an affinity constant of 23.9 nM as determined in a SPR assay (Routinely tested).
|Gene Name :||IL7RA|
|Official Symbol :||IL7RA|
|Synonyms :||ILRA; CD127; IL7RA; CDW127; IL-7R-alpha|
|Gene ID :||3575|
|mRNA Refseq :||NM_002185.5|
|Protein Refseq :||NP_002176.2|
|UniProt ID :||P16871|
◆ Recombinant Protein
|IL7RA-324H||Recombinant Human IL7RA protein, Mouse IgG2a Fc-tagged||+Inquiry|