Recombinant Human IL7RA protein, Mouse IgG2a Fc-tagged IL7RA-324H

Recombinant Human IL7RA protein, Mouse IgG2a Fc-tagged

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Cat.No. : IL7RA-324H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human IL7RA protein (Glu21-Gly236), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra/CD127 contains 1 fibronectin type-III domain. IL7Ra/CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra/CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.
Source : HEK293
Species : Human
Tag : Mouse IgG2a Fc
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 52.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 216
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IL7RA-324H :

Human IL-7 R alpha, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of IL7RA-324H :

Immobilized Human IL-7, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 4-31 ng/mL (QC tested).

Biotinylated Human TSLP (R127A, R130A), His,Avitag immobilized at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag , can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 1-20 ng/mL (Routinely tested).

Bioactivity-SPR of IL7RA-324H :

Captured Human IL-7R alpha, Mouse IgG2a Fc Tag on CM5 chip via anti-mouse antibodies surface can bind Human IL-7, Tag Free with an affinity constant of 13.3 nM as determined in a SPR assay (Routinely tested).

Gene Name : IL7RA
Official Symbol : IL7RA
Synonyms : ILRA; CD127; IL7RA; CDW127; IL-7R-alpha
Gene ID : 3575
mRNA Refseq : NM_002185.5
Protein Refseq : NP_002176.2
MIM : 146661
UniProt ID : P16871
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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