Active Recombinant Human INSR Protein, His-tagged

• Cat.No.: INSR-622H
• Product Overview: Active Recombinant Human INSR Protein(NP_001073285.1)(Met 1-Lys 944) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-944 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. When 1 μg/ml of biotinylated human insulin is immobilized onto a streptavidin coated plate, it can bind human insulin receptor with a linear range of 0.3-40 μg/ml.
• Molecular Mass: The secreted recombinant human INSR isoform short consists of 928 amino acids and has a predicted molecular mass of 106 (83+23) kDa. As a result of glycosylation, the apparent molecular mass of rhINSR is approximately 125-135 kDa & 40-45 kDa, corresponding to the α subunit and the ECD of β subunit respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_000199.2
• Unit ID: P06213
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P06213

Gene Information

• Gene Name: INSR insulin receptor [ Homo sapiens ]
• Official Symbol: INSR
• Synonyms: INSR; insulin receptor; CD220; IR; HHF5
• Gene ID: 3643
• mRNA Refseq: NM_000208
• protein Refseq: NP_000199
• MIM: 147670