Recombinant Human INSR protein, His-tagged
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|Product Overview :||Recombinant Human INSR (His28-Lys944) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.|
|Predicted N Terminal :||His28|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 106.5 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||His28-Lys944|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of INSR-29H :||
Human Insulin R (28-944), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of INSR-29H :||
Immobilized Human Insulin R (28-944), His Tag at 5 μg/mL (100 μL/well)can bind Human IGF-II, Fc Tag with a linear range of 0.078-1.25 μg/mL (QC tested).
Immobilized Human Insulin R (28-944), His Tag at 5 μg/mL (100 μL/well)can bind Insulin Biotin Conjugate with a linear range of 0.02-0.313 μg/mL (Routinely tested).
|Bioactivity-SPR of INSR-29H :||
Human Insulin R (28-944), His Tag captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nM as determined in a SPR assay (Biacore T200) (QC tested).
|Gene Name :||INSR|
|Official Symbol :||INSR|
|Synonyms :||INSR; insulin receptor; CD220; IR; HHF5|
|Gene ID :||3643|
|mRNA Refseq :||NM_000208|
|Protein Refseq :||NP_000199|
|UniProt ID :||P06213|
◆ Recombinant Protein
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|INSR-5092H||Recombinant Human INSR Protein, GST-tagged||+Inquiry|
|INSR-1260H||Recombinant Human INSR Protein, His-SUMO-tagged||+Inquiry|
|INSR-29493TH||Recombinant Human INSR||+Inquiry|
|INSR-2648HCL||Recombinant Human INSR cell lysate||+Inquiry|
|INSR-474HCL||Recombinant Human INSR cell lysate||+Inquiry|
◆ Assay kits
|Kit-1607||INSR Internalization Assay Kit||+Inquiry|
|Kit-1605||U2OS INSR Bioassay Kit||+Inquiry|