Recombinant Human INSR protein, His-tagged

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Recombinant Human INSR protein, His-tagged

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Cat.No. : INSR-29H
Product Overview : Recombinant Human INSR (His28-Lys944) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : His28
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 106.5 kDa. The protein migrates as 115-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : His28-Lys944
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of INSR-29H :

Human Insulin R (28-944), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of INSR-29H :

Immobilized Human Insulin R (28-944), His Tag at 5 μg/mL (100 μL/well)can bind Human IGF-II, Fc Tag with a linear range of 0.078-1.25 μg/mL (QC tested).

Bioactivity-ELISA :

Immobilized Human Insulin R (28-944), His Tag at 5 μg/mL (100 μL/well)can bind Insulin Biotin Conjugate with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-SPR of INSR-29H :

Human Insulin R (28-944), His Tag captured on CM5 chip via anti-His antibody, can bind Human Insulin with an affinity constant of 249 nM as determined in a SPR assay (Biacore T200) (QC tested).

Gene Name : INSR
Official Symbol : INSR
Synonyms : INSR; insulin receptor; CD220; IR; HHF5
Gene ID : 3643
mRNA Refseq : NM_000208
Protein Refseq : NP_000199
MIM : 147670
UniProt ID : P06213

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