| Cat. No. : |
IL17A-26H |
| Description : |
The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 132 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Formulation : |
Sterile filtered lyophilized powder, with 20mM Citrate, 0.1M NaCl, pH4.5. |
| Specific Activity : |
The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2ng/ml, corresponding to a specific activity of 5.0 x 105IU/mg. |
| Endotoxin : |
Less than 1 IEU/µg determined by LAL test. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized rHuIL-17 in sterile 18MΩ-H2O containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of no less than 100ug/ml of the cytokine. |
| Storage : |
Lyophilized rHuIL-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-17 should be stored at 4°C between 2-7 days and for future use below -18°C. Aliquot to avoid repeated freeze-thaw cycles. |
