| Description : |
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
118.7 kDa |
| AA Sequence : |
MESAGLEQLLRELLLPDTERIRRAT EQLQIVLRAPAALPALCDLLASAAD PQIRQFAAVLTRRRLNTRWRRLAAE QRESLKSLILTALQRETEHCVSLSL AQLSATIFRKEGLEAWPQLLQLLQH STHSPHSPEREMGLLLLSVVVTSRP EAFQPHHRELLRLLNETLGEVGSPG LLFYSLRTLTTMAPYLSTEDVPLAR MLVPKLIMAMQTLIPIDEAKACEAL EALDELLESEVPVITPYLSEVLTFC LEVARNVALGNAIRIRILCCLTFLV KVKSKALLKNRLLPPLLHTLFPIVA AEPPPGQLDPEDQDSEEEELEIELM GETPKHFAVQVVDMLALHLPPEKLC PQLMPMLEEALRSESPYQRKAGLLV LAVLSDGAGDHIRQRLLPPLLQIVC KGLEDPSQVVRNAALFALGQFSENL QPHISSYSREVMPLLLAYLKSVPLG HTHHLAKACYALENFVENLGPKVQP YLPELMECMLQLLRNPSSPRAKELA VSALGAIATAAQASLLPYFPAIMEH LREFLLTGREDLQPVQIQSLETLGV LARAVGEPMRPLAEECCQLGLGLCD QVDDPDLRRCTYSLFAALSGLMGEG LAPHLEQITTLMLLSLRSTEGIVPQ YDGSSSFLLFDDESDGEEEEELMDE DVEEEDDSEISGYSVENAFFDEKED TCAAVGEISVNTSVAFLPYMESVFE EVFKLLECPHLNVRKAAHEALGQFC CALHKACQSCPSEPNTAALQAALAR VVPSYMQAVNRERERQVVMAVLEAL TGVLRSCGTLTLKPPGRLAELCGVL KAVLQRKTACQDTDEEEEEEDDDQA EYDAMLLEHAGEAIPALAAAAGGDS FAPFFAGFLPLLVCKTKQGCTVAEK SFAVGTLAETIQGLGAASAQFVSRL LPVLLSTAQEADPEVRSNAIFGMGV LAEHGGHPAQEHFPKLLGLLFPLLA RERHDRVRDNICGALARLLMASPTR KPEPQVLAALLHALPLKEDLEEWVT IGRLFSFLYQSSPDQVIDVAPELLR ICSLILADNKIPPDTKAALLLLLTF LAKQHTDSFQAALGSLPVDKAQELQ AVLGLSTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
