Recombinant Human IRAK2 293 Cell Lysate

• Cat.No.: IRAK2-5171HCL

Specification

• Description: Antigen standard for interleukin-1 receptor-associated kinase 2 (IRAK2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: IRAK2 interleukin-1 receptor-associated kinase 2 [ Homo sapiens ]
• Official Symbol: IRAK2
• Synonyms: IRAK2; interleukin-1 receptor-associated kinase 2; interleukin-1 receptor-associated kinase-like 2; interleukin-1 receptor associated kinase-2; IRAK-2; MGC150550
• Gene ID: 3656
• mRNA Refseq: NM_001570
• Protein Refseq: NP_001561
• MIM: 603304
• UniProt ID: O43187
• Chromosome Location: 3p25.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem; IRAK2 mediated activation of TAK1 complex, organism-specific biosystem; IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; transferase activity, transferring phosphorus-containing groups

Reviews

11/23/2021

Trusting in its consistent results, I am able to embark on my research with assurance and optimism.

08/22/2018

Its unwavering stability and functionality have significantly contributed to the success and validity of my experimental outcomes.

10/22/2016

I am profoundly impressed by the technical support rendered by the manufacturer.

Q&As

Are there any clinical trials targeting IRAK2?

Yes, there are ongoing clinical trials exploring IRAK2 inhibitors as potential treatments for autoimmune diseases and other conditions.

Is there a diagnostic test for IRAK2-related conditions?

Currently, there are no widely available diagnostic tests specifically for IRAK2-related conditions. Diagnosis often relies on clinical symptoms and other tests.

How is IRAK2 involved in inflammatory responses?

IRAK2 is a key player in the inflammatory signaling pathway, which can lead to the production of pro-inflammatory cytokines.

Are there any cancer treatments targeting IRAK2?

How can gene mutations in IRAK2 affect human health?

Gene mutations in IRAK2 can disrupt the normal immune response, leading to susceptibility to infections and autoimmune diseases.