Recombinant Human ISG15 Protein, GST-tagged

• Cat.No.: ISG15-987H
• Product Overview: Recombinant Human ISG15 Protein(NP_005092.1)(1-165 aa) is expressed from E. coli with a GST Tag at the N-terminal.

Specification

• Description: The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Bio-activity: Not tested.
• Protein length: 1-165 aa
• AA Sequence: MGWDLTVKMLAGNEFQVSLSSSMSV SELKAQITQKIGVHAFQQRLAVHPS GVALQDRVPLASQGLGPGSTVLLVV DKCDEPLNILVRNNKGRSSTYEVRL TQTVAHLKQQVSGLEGVQDDLFWLT FEGKPLEDQLPLGEYGLKPLSTVFM NLRLRGGGTEPGGRS
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Applications: Blocking peptide
• Stability: Store for up to 12 months at -20°C to -80°C as lyophilized powder.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).

Gene Information

• Gene Name: ISG15
• Official Symbol: ISG15
• Synonyms: G1P2; IFI15; UCRP
• Gene ID: 9636
• mRNA Refseq: NM_005101.4
• Protein Refseq: NP_005092.1
• MIM: 147571
• UniProt ID: P05161