| Cat.No. : |
ITGA5&ITGB1-358H |
| Product Overview : |
Recombinant Human ITGA5 & ITGB1 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)). Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1). Human ITGA5 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 104.1 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 117-150 kDa (ITGA5) and 100-116 kDa (ITGB1) respectively due to glycosylation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Recombinant Human ITGA5 & ITGB1 Heterodimer Protein, His-tagged with a linear range of 0.2-2 ng/mL (QC tested). |
| Molecular Mass : |
104.1 kDa (ITGA5) & 83.7 kDa (ITGB1) |
| Protein length : |
Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
|
