Active Recombinant Human ITGA5 & ITGB1 Heterodimer Protein, His-tagged

• Cat.No.: ITGA5&ITGB1-358H
• Product Overview: Recombinant Human ITGA5 & ITGB1 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)). Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1). Human ITGA5 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 104.1 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 117-150 kDa (ITGA5) and 100-116 kDa (ITGB1) respectively due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Recombinant Human ITGA5 & ITGB1 Heterodimer Protein, His-tagged with a linear range of 0.2-2 ng/mL (QC tested).
• Molecular Mass: 104.1 kDa (ITGA5) & 83.7 kDa (ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGA5 & ITGB1
• Synonyms: ITGA5; FNRA; integrin alpha-5; CD49e; VLA-5; integrin alpha-F; VLA5A; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 3678 & 3688
• mRNA Refseq: NM_002205 & NM_002211
• Protein Refseq: NP_002196 & NP_002202
• MIM: 135620 & 135630
• UniProt ID: P08648 & P05556