| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His-Avi |
| Predicted N Terminal : |
Phe 31 (ITGAV) & Gly 24 (ITGB5) |
| Form : |
Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose. |
| Bio-activity : |
Immobilized Human Vitronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein with a linear range of 0.01-0.156 ug/mL. Immobilized Human Fibronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein with a linear range of 0.156-5 ug/mL. |
| Molecular Mass : |
Biotinylated Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). The reducing protein migrates as 120 kDa and 150kDa (ITGAV) and 90 kDa (ITGB5) respectively due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Characteristic : |
Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Official Symbol : |
ITGAV; ITGB5 |
| Synonyms : |
Integrin alpha V beta 5, ITGAV & ITGB5 |
