Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
1-316 a.a. |
Description : |
JMJD7, also known as JmjC domain-containing protein 7, belongs to the JMJD family. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD7 has not been identified. It was initially thought to be a novel splice form of the phospholipase PLA2G4B which is located downstream of JMJD7 as most tissues express a read-through transcript of the two genes. Recombinant human JMJD7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form : |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl |
Molecular Mass : |
38.3 kDa(339aa) confirmed by MALDI-TOF |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMAEAALE AVRSELREFP AAARELCVPL AVPYLDKPPT PLHFYRDWVC PNRPCIIRNA LQHWPALQKW SLPYFRATVG STEVSVAVTP DGYADAVRGD RFMMPAERRL PLSFVLDVLE GRAQHPGVLY VQKQCSNLPS ELPQLLPDLE SHVPWASEAL GKMPDAVNFW LGEAAAVTSL HKDHYENLYC VVSGEKHFLF HPPSDRPFIP YELYTPATYQ LTEEGTFKVV DEEAMEKVPW IPLDPLAPDL ARYPSYSQAQ ALRCTVRAGE MLYLPALWFH HVQQSQGCIA VNFWYDMEYD LKYSYFQLLD SLTKASGLD |
Purity : |
>90% by SDS - PAGE |
Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : |
1mg/ml (determined by Bradford assay) |