Recombinant Human KAT2A Protein, His-tagged KAT2A-005H

Recombinant Human KAT2A Protein, His-tagged

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Recombinant Human KAT2A Protein, His-tagged

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Cat.No. : KAT2A-005H
Product Overview : Recombinant Human KAT2A protein (2-837)with a N-terminal His-tag was expressed in Sf21 cells
Description : KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT-independent manner.
Source : Insect Cells
Species : Human
Tag : His
Molecular Mass : 96.3 kDa
Protein length : 2-837
Purity : ≥50 % (estimated by SDS-PAGE)
Unit Definition : One unit is defined as the amount of enzyme required to convert 1 nmol of acetyl-CoA to reduced Coenzyme A (CoASH) per minute at 37 centigrade in 100 mM HEPES, pH 7.5, containing 0.8% polysorbate 20. CoASH produced in the reaction is measured fluorometrically using HAT Inhibitor Screening Assay Kit with 100 µM histone H3 peptide (1-21) as the acetyl acceptor.
Stability : ≥ 2 years
Storage : -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
Gcn5 enzyme titration :

Histone H3 (1-21) peptide titration :

SDS-PAGE analysis of KAT2A. :

Lane 1: MW Markers
Lane 2: Gcn5 (1 µg)
Lane 3: Gcn5 (2 µg)
Lane 4: Gcn5 (4 µg)

Gene Name : KAT2A lysine acetyltransferase 2A [ Homo sapiens (human) ]
Official Symbol : KAT2A
Synonyms : KAT2A; lysine acetyltransferase 2A; GCN5; hGCN5; GCN5L2; PCAF-b; histone acetyltransferase KAT2A; GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; General control of amino acid synthesis, yeast, homolog-like 2 K(lysine) acetyltransferase 2A; STAF97; general control of amino acid synthesis protein 5-like 2; histone acetyltransferase GCN5; histone glutaryltransferase KAT2A; histone succinyltransferase KAT2A; hsGCN5; EC 2.3.1.48
Gene ID : 2648
mRNA Refseq : NM_021078
Protein Refseq : NP_066564
MIM : 602301
UniProt ID : Q92830

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