Recombinant Human Keratin 8

• Cat.No.: KRT8-1247H
• Product Overview: Recombinant humanKRT8 is a single, non-glycosylated polypeptide chain.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: KRT8 also known ascytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human isencoded by the KRT8 gene. It is often paired with keratin 18. KRT8 can beused to differentiate lobular carcinoma of the breast from ductal carcinomaof the breast. KRT8 is often used together with keratin 18 and keratin 19 todifferentiate cells of epithelial origin from hematopoietic cells in teststhat enumerate circulating tumor cells in blood.
• Form: Lyophilized from asterile filtered solution containing 30 mM Tris-HCl, pH 8.0 + 9.5 M urea + 2mM DTT + 2 mM EDTA + 10 mM methylammonium chloride.
• MolecularWeight: 53.5 kDa
• Purity: > 95% asdetermined by RP-HPLC and SDS-PAGE analyses
• Endotoxin Level: < 0.1 ng/µg ofKRT8
• Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fully solubilizethe protein to a concentration not less than 100 µg/ml. After complete solubilizationof the protein, it can be further diluted to other aqueous solutions.
• Storage: Store lyophilizedprotein at -20°C to -80°C. Reconstituted protein is stable for 1 week at 2-4°C.For long term storage, aliquot and store at -20°C to -80°C with a carrierprotein (0.1% HSA or BSA) as a stabilizer. Please note that the addition ofany carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Avoid repeated freeze-thawcycles.
• OfficialSymbol: KRT8

Gene Information

• Gene Name: KRT8 keratin 8 [ Homo sapiens]
• Synonyms: KRT8; keratin 8; K8;KO; CK8; CK-8; CYK8; K2C8; CARD2; keratin, type II cytoskeletal 8; Type-IIkeratin Kb8; keratin-8; Keratin-8; cytokeratin 8; cytokeratin-8;OTTHUMP00000242366; OTTHUMP00000242367; OTTHUMP00000242368;OTTHUMP00000242369; OTTHUMP00000242370; type-II keratin Kb8
• Gene ID: 3856
• mRNA Refseq: NM_002273
• Protein Refseq: NP_002264
• MIM: 148060
• UniProt ID: P05787
• Chromosome Location: 12q13
• Pathway: EGFR1 SignalingPathway; Signaling mediated by p38-alpha and p38-beta
• Function: protein binding;structural molecule activity