Recombinant Human KRT8 293 Cell Lysate
Cat.No. : | KRT8-4862HCL |
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Description : | Antigen standard for keratin 8 (KRT8) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | KRT8 keratin 8 [ Homo sapiens ] |
Official Symbol : | KRT8 |
Synonyms : | KRT8; keratin 8; keratin, type II cytoskeletal 8; CARD2; CK8; CYK8; K2C8; K8; KO; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; CK-8; |
Gene ID : | 3856 |
mRNA Refseq : | NM_001256282 |
Protein Refseq : | NP_001243211 |
MIM : | 148060 |
UniProt ID : | P05787 |
Chromosome Location : | 12q13 |
Pathway : | EGFR1 Signaling Pathway, organism-specific biosystem; Signaling mediated by p38-alpha and p38-beta, organism-specific biosystem; |
Function : | protein binding; structural molecule activity; |
Products Types
◆ Recombinant Protein | ||
KRT8-2562H | Recombinant Human KRT8 protein(71-410 aa), C-His-tagged | +Inquiry |
KRT8-2988R | Recombinant Rat KRT8 Protein, His (Fc)-Avi-tagged | +Inquiry |
Krt8-1285M | Recombinant Mouse Krt8 Protein, MYC/DDK-tagged | +Inquiry |
KRT8-2269R | Recombinant Rhesus Macaque KRT8 Protein, His (Fc)-Avi-tagged | +Inquiry |
KRT8-1267H | Recombinant Human KRT8 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
KRT8-177B | Native bovine KRT8 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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