Active Recombinant Human KLK3 protein(Primary Amine Labeling), His-tagged, Biotinylated

• Cat.No.: KLK3-1258H
• Product Overview: Biotinylated Active Recombinant Human KLK3 protein(Primary Amine Labeling)(P07288-1)(Ile25-Pro261) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-261 aa
• Conjugation/Label: Biotin; Primary Amine Labeling
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl, 8% trehalose, 0.05% Brij-35 (pH 7.5).
• Bio-activity: Immobilized Biotinylated Human Kallikrein 3, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Kallikrein 3 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 31-38 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK3 kallikrein-related peptidase 3 [ Homo sapiens ]
• Official Symbol: KLK3
• Synonyms: KLK3; kallikrein-related peptidase 3; APS, kallikrein 3, (prostate specific antigen); prostate-specific antigen; PSA; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen; APS; hK3; KLK2A1
• Gene ID: 354
• mRNA Refseq: NM_001030047
• Protein Refseq: NP_001025218
• MIM: 176820
• UniProt ID: P07288