Recombinant Human KLK5 protein, His-tagged

• Cat.No.: KLK5-5792H
• Product Overview: Recombinant Human KLK5 protein(Q9Y337)(Val23-Ser293), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Val23-Ser293
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5).
• Bio-activity: Immobilized Human Kallikrein 5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Kallikrein 5 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 42-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLK5 kallikrein-related peptidase 5 [ Homo sapiens ]
• Official Symbol: KLK5
• Synonyms: KLK5; kallikrein-related peptidase 5; kallikrein 5; kallikrein-5; KLK L2; SCTE; kallikrein-like protein 2; stratum corneum tryptic enzyme; KLKL2; KLK-L2
• Gene ID: 25818
• mRNA Refseq: NM_001077491
• Protein Refseq: NP_001070959
• MIM: 605643
• UniProt ID: Q9Y337