Active Recombinant Human KLK7 protein, His-tagged, Low Endotoxin

• Cat.No.: KLK7-893H
• Product Overview: Recombinant Human KLK7 protein(P49862-1)(Glu23-Arg253), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 23-253 aa
• Form: Lyophilized from 0.22 μm filtered solution in 20mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Kallikrein 7, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kallikrein 7 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.81 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: KLK7 kallikrein-related peptidase 7 [ Homo sapiens ]
• Official Symbol: KLK7
• Synonyms: KLK7; kallikrein-related peptidase 7; kallikrein 7 (chymotryptic, stratum corneum), PRSS6; kallikrein-7; SCCE; signal protein; serine protease 6; stratum corneum chymotryptic enzyme; kallikrein 7 (chymotryptic, stratum corneum); hK7; PRSS6
• Gene ID: 5650
• mRNA Refseq: NM_001243126
• Protein Refseq: NP_001230055
• MIM: 604438
• UniProt ID: P49862