Recombinant Human KLRC2&KLRD1 Heterodimer Protein, His/FLAG-tagged

• Cat.No.: KLRC2&KLRD1-162H
• Product Overview: Recombinant Human NKG2C/CD94 is produced by Human 293 Cells. The target gene encoding E98-L231& S34-I179 is expressed with a 8His & a FLAG tag at the N terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 98-231;34-179 a.a.
• Description: NKG2-C contains C-type lectin domain and belongs to the killer cell lectin-like receptor (KLR) family. KLR family is a group of transmembrane proteins preferentially expressed in natural killer (NK) cells. NK cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. CD94, also known as killer cell lectin-like receptor subfamily D member 1 (KLRD1), is expressed on the surface of NK cells in the innate immune system. CD94 plays a role as a receptor for the recognition of MHC class I Human Leukocyte Antigen (HLA)-E molecules by NK cells and some cytotoxic T-cells. CD94 can form disulfide-bonded heterodimer with NKG2C on the surface of NK cells. The CD94/NKG2C complex interacts with HLA-E on target cells and inhibit the cytotoxic activity of NK cells to prevent cell lysis.
• Form: Lyophilized frum a 0.2 um filtered solution of PBS, pH7.4.
• Molecular Mass: Predicted Molecular Weight: 18.6kDa & 20.1kDa
  Apparent Molecular Weight: NKG2C protein migrates as 26kDa in an reducing SDS-PAGE gel due to glycosylation. CD94 protein appeared in a smear between 26~34kDa in a reducing SDS-PAGE gel due to glycosylation.
• AA Sequence: E98-L231; S34-I179
• Endotoxin: Less than 0.1 EU/ug.
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: KLRC2 killer cell lectin like receptor C2 [ Homo sapiens (human) ]&KLRD1 killer cell lectin like receptor D1 [ Homo sapiens (human) ]
• Official Symbol: KLRC2&KLRD1
• Synonyms: NKG2C & CD94
• Gene ID: 3822&3824
• mRNA Refseq: NM_002260.4&NM_001114396.3
• Protein Refseq: NP_002251.2&NP_001107868.2
• MIM: 602891&602894
• UniProt ID: P26717 & Q13241