Recombinant Human KLRC2&KLRD1 protein, His-Avi-tagged

• Cat.No.: KLRC2&KLRD1-241H
• Product Overview: Recombinant Human KLRC2&KLRD1 protein(P26717(NKG2C)&Q13241-1(CD94))(Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
• Tag: N-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• GeneID 2: 3824

Gene Information

• Gene Name: KLRC2 killer cell lectin-like receptor subfamily C, member 2 [ Homo sapiens ]
• Official Symbol: KLRC2
• Synonyms: KLRC2; killer cell lectin-like receptor subfamily C, member 2; NKG2-C type II integral membrane protein; CD159c; NKG2 C; NK cell receptor C; NKG2-C-activating NK receptor; CD159 antigen-like family member C; NKG2C; NKG2-C; MGC138244
• Gene ID: 3822
• mRNA Refseq: NM_002260
• Protein Refseq: NP_002251
• MIM: 602891
• UniProt ID: P26717
• Gene Name 2: KLRD1 killer cell lectin-like receptor subfamily D, member 1 [ Homo sapiens ]
• Official Symbol 2: KLRD1
• Synonyms 2: KLRD1; killer cell lectin-like receptor subfamily D, member 1; CD94; natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor
• mRNA Refseq 2: NM_001114396
• Protein Refseq 2: NP_001107868
• MIM 2: 602894
• UniProt ID 2: Q13241