| Cat.No. : |
KMT2A-002H |
| Product Overview : |
Recombinant C-terminal fragment of MLL1, residues 3762 - 3969, contains the SET1 domain for methyltransferase activity, and the WIN motif for binding the WAR complex without tag was expressed in E. coli. (An N-terminal hexahistidine tag and SUMOpro tag were removed by cleavage with SUMO protease 1) |
| Description : |
This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants. |
| Source : |
E. coli |
| Species : |
Human |
| Molecular Mass : |
24.1 kDa |
| Purity : |
≥ 90% estimated by SDS-PAGE |
| Stability : |
≥ 6 months |
| Storage : |
Store at -80 centigrade |
| Storage Buffer : |
50 mM Tris, pH 8.0, with 400 nM sodium chloride, 10% glycerol, and 5 mM 2-mercaptoethanol |
| Shipping : |
Dry ice in continental US, may vary elsewhere. |
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