Recombinant Human KNSTRN Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: KNSTRN-277H
• Product Overview: C15orf23 MS Standard C13 and N15-labeled recombinant protein (NP_150628) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Essential component of the mitotic spindle required for faithful chromosome segregation and progression into anaphase. Promotes the metaphase-to-anaphase transition and is required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Required for kinetochore oscillations and dynamics of microtubule plus-ends during live cell mitosis, possibly by forming a link between spindle microtubule plus-ends and mitotic chromosomes to achieve faithful cell division. May be involved in UV-induced apoptosis via its interaction with PRPF19; however, these results need additional evidences.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 35.4 kDa
• AA Sequence: MAAPEAPPLDRVFRTTWLSTECDSH PLPPSYRKFLFETQEADLAGGTTVA AGNLLNESEKDCGQDRRAPGVQPCL LVTMTSVVKTVYSLQPSSALSGGQP ADTQTRATSKSLLPVRSKEVDVSKQ LHSGGPENDVTKITKLRRENGQMKA TDTATRRNVRKGYKPLSKQKSEEEL KDKNQLLEAVNKQLHQKLTETQGEL KDLTQKVELLEKFRDNCLAILESKG LDPALGGETLASRQESTTDHMDSML LLETLQEELKLFNETAKKQMEELQA LKVKLEMKEERVRFLEQQTLCNNQV NDLTTALKEMEQLLEMTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: KNSTRN kinetochore localized astrin (SPAG5) binding protein [ Homo sapiens (human) ]
• Official Symbol: KNSTRN
• Synonyms: KNSTRN; kinetochore localized astrin (SPAG5) binding protein; SKAP; HSD11; C15orf23; TRAF4AF1; small kinetochore-associated protein; TRAF4 associated factor 1; kinastrin; kinetochore-localized astrin-binding protein; putative TRAF4-associated factor 1; small kinetochore associated protein; small kinetochore-associated protein, kinetochore-localized astrin-binding protein, TRAF4 associated factor 1
• Gene ID: 90417
• mRNA Refseq: NM_033286
• Protein Refseq: NP_150628
• MIM: 614718
• UniProt ID: Q9Y448