Recombinant Human KRT3 protein, His & T7-tagged

• Cat.No.: KRT3-2696H
• Product Overview: Recombinant Human KRT3 aa. (Glu198~Ser514 (Accession # P12035)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.3kDa
• Protein length: Glu198~Ser514 (Accession # P12035)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.3.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: KRT3 keratin 3 [ Homo sapiens (human) ]
• Official Symbol: KRT3
• Synonyms: K3; CK3; 65 kDa cytokeratin; CK-3; cytokeratin 3; keratin 3, type II; type-II keratin Kb3; keratin, type II cytoskeletal 3
• Gene ID: 3850
• mRNA Refseq: NM_057088.2
• Protein Refseq: NP_476429.2
• UniProt ID: P12035