Recombinant Human KRT79 293 Cell Lysate

Cat.No. : KRT79-4863HCL
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Description : Antigen standard for keratin 79 (KRT79) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : KRT79 keratin 79 [ Homo sapiens ]
Official Symbol : KRT79
Synonyms : KRT79; keratin 79; keratin, type II cytoskeletal 79; K6L; keratin 6 like; KRT6L; K79; CK-79; keratin 6L; keratin-6L; keratin-79; cytokeratin-79; type-II keratin Kb38; FLJ26646;
Gene ID : 338785
mRNA Refseq : NM_175834
Protein Refseq : NP_787028
MIM : 611160
UniProt ID : Q5XKE5
Chromosome Location : 12q13.13
Function : structural molecule activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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