Recombinant Human KRT9 protein, His-tagged

• Cat.No.: KRT9-2782H
• Product Overview: Recombinant Human KRT9 aa. (Pro315~Asn456) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro315~Asn456
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >92%
• Characteristic: The isoelectric point is 5.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: KRT9 keratin 9 [ Homo sapiens (human) ]
• Official Symbol: KRT9
• Synonyms: K9; CK-9; EPPK; cytokeratin 9; keratin 9, type I; type I cytoskeletal 9; keratin, type I cytoskeletal 9
• Gene ID: 3857
• mRNA Refseq: NM_000226.3
• Protein Refseq: NP_000217.2
• UniProt ID: P35527