Active Recombinant Human LCN1 protein, His-tagged

• Cat.No.: LCN1-3216H
• Product Overview: Active Recombinant Human LCN1 protein(NP_002288.1)(Met 1-Asp176) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-176 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value is < 400 nM.
• Molecular Mass: The secreted recombinant human LCN1 consists of 169 amino acids and has a predicted molecular mass of 19 kDa The apparent molecular mass of the protein is approximately 20 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LCN1 lipocalin 1 [ Homo sapiens ]
• Official Symbol: LCN1
• Synonyms: LCN1; lipocalin 1; lipocalin 1 (protein migrating faster than albumin, tear prealbumin), lipocalin 1 (tear prealbumin); lipocalin-1; lipocalin 1 like 2; MGC71975; PMFA; tear lipocalin; tear prealbumin; TLC; TP; VEGP; Von Ebner gland protein; VEG protein; lipocalin 1 (tear prealbumin); protein migrating faster than albumin
• Gene ID: 3933
• mRNA Refseq: NM_001252618
• Protein Refseq: NP_001239547
• MIM: 151675
• UniProt ID: P31025