Recombinant Human LEFTY2 protein, His-tagged

• Cat.No.: LEFTY2-501H
• Product Overview: Recombinant Human LEFTY2 protein(NP_003231)(24-366 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Protein length: 24-366 aa
• AA Sequence: EEQLLGSLLRQLQLSEVPVLDRADM EKLVIPAHVRAQYVVLLRRSHGDRS RGKRFSQSFREVAGRFLASEASTHL LVFGMEQRLPPNSELVQAVLRLFQE PVPKAALHRHGRLSPRSAQARVTVE WLRVRDDGSNRTSLIDSRLVSVHES GWKAFDVTEAVNFWQQLSRPRQPLL LQVSVQREHLGPLASGAHKLVRFAS QGAPAGLGEPQLELHTLDLRDYGAQ GDCDPEAPMTEGTRCCRQEMYIDLQ GMKWAKNWVLEPPGFLAYECVGTCQ QPPEALAFNWPFLGPRQCIASETAS LPMIVSIKEGGRTRPQVVSLPNMRV QKCSCASDGALVPRRLQP
• Purity: 70%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Gene Information

• Gene Name: LEFTY2 left-right determination factor 2 [ Homo sapiens ]
• Official Symbol: LEFTY2
• Synonyms: LEFTY2; left-right determination factor 2; EBAF, endometrial bleeding associated factor (left right determination, factor A; transforming growth factor beta superfamily) , TGFB4; LEFTA; LEFTYA; transforming growth factor; beta 4 (endometrial bleeding associated factor; LEFTY A); TGF-beta-4; protein lefty-2; protein lefty-A; left-right determination factor A; transforming growth factor beta-4; endometrial bleeding-associated factor; EBAF; TGFB4; MGC46222
• Gene ID: 7044
• mRNA Refseq: NM_003240
• Protein Refseq: NP_003231
• UniProt ID: O00292