Recombinant Human LEP protein

• Cat.No.: LEP-189H
• Product Overview: Recombinant Human LEP was expressed in E. coli.

Specification

• Description: Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Molecular Mass: 16.4 kDa
• Endotoxin: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: 95%
• Applications: Cell Assay
• Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade- 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Reconstitution: Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCl (pH 8.0).

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; leptin (murine obesity homolog) , leptin (obesity homolog, mouse) , OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• UniProt ID: P41159
• Chromosome Location: 7q31
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem
• Function: growth factor activity; hormone activity; peptide hormone receptor binding