Recombinant Human LGALS9 Protein (1-323 aa), His-tagged

• Cat.No.: LGALS9-2145H
• Product Overview: Recombinant Human LGALS9 Protein (1-323 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cell Biology. Protein Description: Full Length of Isoform 2.

Specification

• Description: Binds galactosides (PubMed:18005988). Has high affinity for the Forssman pentasaccharide (PubMed:18005988). Ligand for HAVCR2/TIM3 (PubMed:16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed:16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed:23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed:20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed:24465902, PubMed:16116184). Inhibits degranulation and induces apoptosis of mast cells (PubMed:24465902). Induces maturation and migration of dendritic cells (PubMed:25754930, PubMed:16116184). Inhibits natural killer (NK) cell function (PubMed:23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed:25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity).By similarity11 PublicationsIsoform 2: Acts as an eosinophil chemoattractant (PubMed:9642261). It also inhibits angiogenesis (PubMed:24333696). Suppresses IFNG production by natural killer cells (By similarity).
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 37.7 kDa
• Protein length: 1-323 aa
• AA Sequence: MAFSGSQAPYLSPAVPFSGTIQGGL QDGLQITVNGTVLSSSGTRFAVNFQ TGFSGNDIAFHFNPRFEDGGYVVCN TRQNGSWGPEERKTHMPFQKGMPFD LCFLVQSSDFKVMVNGILFVQYFHR VPFHRVDTISVNGSVQLSYISFQPP GVWPANPAPITQTVIHTVQSAPGQM FSTPAIPPMMYPHPAYPMPFITTIL GGLYPSKSILLSGTVLPSAQRFHIN LCSGNHIAFHLNPRFDENAVVRNTQ IDNSWGSEERSLPRKMPFVRGQSFS VWILCEAHCLKVAVDGQHLFEYYHR LRNLPTINRLEVGGDIQLTHVQT
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: LGALS9 lectin, galactoside-binding, soluble, 9 [ Homo sapiens ]
• Official Symbol: LGALS9
• Synonyms: LGALS9; galectin-9; galectin 9; LGALS9A; gal-9; ecalectin; HUAT; MGC117375; MGC125973; MGC125974
• Gene ID: 3965
• mRNA Refseq: NM_002308
• Protein Refseq: NP_002299
• MIM: 601879
• UniProt ID: O00182