Recombinant Human LIN28B Protein (2-250), N-FLAG/Avi-tagged, Biotin-labeled

• Cat.No.: LIN28B-09H
• Product Overview: Human LIN28B also known as Lin-28 Homolog B, with N-terminal FLAG-Avi-Tag and biotin labeled, GenBank Accession No. NM_001004317, a.a. 2-250 (end), expressed in a HEK293 cell expression system.

Specification

• Description: The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation.
• Source: HEK293
• Species: Human
• Tag: N-FLAG and Avi
• Form: Aqueous buffer solution
• Molecular Mass: 30 kDa
• Protein Length: 2-250
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Notes: The aggregation state of this product has been confirmed to be < 10%.
• Storage: At least 6 months at -80 centigrade
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100µg/ml FLAG peptide, 3mM DTT and 20% glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/mL in PBS + glycerol and stored at -80 centigrade.

Gene Information

• Gene Name: LIN28B lin-28 homolog B [ Homo sapiens (human) ]
• Official Symbol: LIN28B
• Synonyms: LIN28B; lin-28 homolog B; CSDD2; protein lin-28 homolog B; Lin-28.2; lin-28B
• Gene ID: 389421
• mRNA Refseq: NM_001004317
• Protein Refseq: NP_001004317
• MIM: 611044
• UniProt ID: A7E2T3