Recombinant Human LMAN1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: LMAN1-6462H
• Product Overview: LMAN1 MS Standard C13 and N15-labeled recombinant protein (NP_005561) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 57.5 kDa
• AA Sequence: MAGSRQRGLRARVRPLFCALLLSLG RFVRGDGVGGDPAVALPHRRFEYKY SFKGPHLVQSDGTVPFWAHAGNAIP SSDQIRVAPSLKSQRGSVWTKTKAA FENWEVEVTFRVTGRGRIGADGLAI WYAENQGLEGPVFGSADLWNGVGIF FDSFDNDGKKNNPAIVIIGNNGQIH YDHQNDGASQALASCQRDFRNKPYP VRAKITYYQNTLTVMINNGFTPDKN DYEFCAKVENMIIPAQGHFGISAAT GGLADDHDVLSFLTFQLTEPGKEPP TPDKEISEKEKEKYQEEFEHFQQEL DKKKEEFQKGHPDLQGQPAEEIFES VGDRELRQVFEGQNRIHLEIKQLNR QLDMILDEQRRYVSSLTEEISKRGA GMPGQHGQITQQELDTVVKTQHEIL RQVNEMKNSLSETVRLVSGMQHPGS AGGVYETTQHFIDIKEHLHIVKRDI DNLVQRNMPSNEKPKCPELPPFPSC LSTVHFIIFVVVQTVLFIGYIMYRS QQEAAAKKFFTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: LMAN1 lectin, mannose binding 1 [ Homo sapiens (human) ]
• Official Symbol: LMAN1
• Synonyms: LMAN1; lectin, mannose-binding, 1; coagulation factor V factor VIII combined deficiency, F5F8D; protein ERGIC-53; endoplasmic reticulum golgi intermediate compartment protein 53; ERGIC 53; ERGIC53; FMFD1; gp58; MCFD1; MR60; intracellular mannose specific lectin; intracellular mannose-specific lectin MR60; ER-Golgi intermediate compartment 53 kDa protein; endoplasmic reticulum-golgi intermediate compartment protein 53; F5F8D; ERGIC-53
• Gene ID: 3998
• mRNA Refseq: NM_005570
• Protein Refseq: NP_005561
• MIM: 601567
• UniProt ID: P49257