Recombinant Full Length Human LMAN1 Protein, C-Flag-tagged

• Cat.No.: LMAN1-224HFL
• Product Overview: Recombinant Full Length Human LMAN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.2 kDa
• AA Sequence: MAGSRQRGLRARVRPLFCALLLSLG RFVRGDGVGGDPAVALPHRRFEYKY SFKGPHLVQSDGTVPFWAHA GNAIPSSDQIRVAPSLKSQRGSVWT KTKAAFENWEVEVTFRVTGRGRIGA DGLAIWYAENQGLEGPVFGS ADLWNGVGIFFDSFDNDGKKNNPAI VIIGNNGQIHYDHQNDGASQALASC QRDFRNKPYPVRAKITYYQN TLTVMINNGFTPDKNDYEFCAKVEN MIIPAQGHFGISAATGGLADDHDVL SFLTFQLTEPGKEPPTPDKE ISEKEKEKYQEEFEHFQQELDKKKE EFQKGHPDLQGQPAEEIFESVGDRE LRQVFEGQNRIHLEIKQLNR QLDMILDEQRRYVSSLTEEISKRGA GMPGQHGQITQQELDTVVKTQHEIL RQVNEMKNSLSETVRLVSGM QHPGSAGGVYETTQHFIDIKEHLHI VKRDIDNLVQRNMPSNEKPKCPELP PFPSCLSTVHFIIFVVVQTV
  LFIGYIMYRSQQEAAAKKFFTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane

Gene Information

• Gene Name: LMAN1 lectin, mannose binding 1 [ Homo sapiens (human) ]
• Official Symbol: LMAN1
• Synonyms: MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53
• Gene ID: 3998
• mRNA Refseq: NM_005570.4
• Protein Refseq: NP_005561.1
• MIM: 601567
• UniProt ID: P49257