Recombinant Human LMO2 293 Cell Lysate

Cat.No. : LMO2-4710HCL
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Description : Antigen standard for LIM domain only 2 (rhombotin-like 1) (LMO2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : LMO2 LIM domain only 2 (rhombotin-like 1) [ Homo sapiens ]
Official Symbol : LMO2
Synonyms : LMO2; LIM domain only 2 (rhombotin-like 1); RBTNL1; rhombotin-2; RBTN2; RHOM2; rhombotin like 1; T cell translocation gene 2; TTG2; LMO-2; rhombotin 2; rhombotin-like 1; LIM domain only protein 2; T-cell translocation gene 2; cysteine-rich protein TTG-2; T-cell translocation protein 2;
Gene ID : 4005
mRNA Refseq : NM_001142315
Protein Refseq : NP_001135787
MIM : 180385
UniProt ID : P25791
Chromosome Location : 11p13
Pathway : Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function : DNA binding; E-box binding; RNA polymerase II activating transcription factor binding; RNA polymerase II regulatory region sequence-specific DNA binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; bHLH transcription factor binding; chromatin binding; cofactor binding; metal ion binding; protein binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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