Recombinant Human Low Density Lipoprotein Receptor Adaptor Protein 1, His-tagged

• Cat.No.: LDLRAP1-1396H
• Product Overview: Recombinant human LDLRAP1 protein, fused to His-tag atN-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: LDLRAP1(Low density lipoprotein receptor adapter protein 1) is a cytosolic proteinwhich contains a phosphotyrosine binding (PTD) domain. LDLRAP1 is a cytosolic adaptor that couples LDLR to endocyticmachinery. Defects in LDLRAP1are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is adisorder caused by defective internalization of LDL receptors (LDLR) in theliver.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl.
• Molecular Weight: 36.1 kDa (328aa), confirmed by MALDI-TOF.
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MDALKSAGRA LIRSPSLAKQ SWGGGGRHRK LPENWTDTRE TLLEGMLFSLKYLGMTLVEQ PKGEELSAAA IKRIVATAKA SGKKLQKVTL KVSPRGIILT DNLTNQLIEN VSIYRISYCTADKMHDKVFA YIAQSQHNQS LECHAFLCTK RKMAQAVTLT VAQAFKVAFE FWQVSKEEKE KRDKASQEGGDVLGARQDCT PPLKSLVATG NLLDLEETAK APLSTVSANT TNMDEVPRPQ ALSGSSVVWE LDDGLDEAFSRLAQSRTNPQ VLDTGLTAQD MHYAQCLSPV DWDKPDSSGT EQDDLFSF
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: LDLRAP1
• Pathways: Chylomicron-mediatedlipid transport; Endocytosis; LDL-mediated lipid transport; Lipid digestion,mobilization, and transport ;Lipoprotein metabolism; Metabolism of lipids andlipoproteins

Gene Information

• Gene Name: LDLRAP1 low density lipoproteinreceptor adaptor protein 1 [ Homo sapiens ]
• Synonyms: LDLRAP1; low densitylipoprotein receptor adaptor protein 1; ARH; ARH1; ARH2;FHCB1; FHCB2; MGC34705; DKFZp586D0624; OTTHUMP00000008526; LDL receptoradaptor protein; autosomal recessive hypercholesterolemia protein; Lowdensity lipoprotein receptor adaptor protein 1; Autosomal recessivehypercholesterolemia protein
• Gene ID: 26119
• mRNA Refseq: NM_015627
• Protein Refseq: NP_056442
• MIM: 605747
• UniProt ID: Q5SW96
• Chromosome Location: 1p36-p35