| Species : |
Human |
| Source : |
Mouse myeloma cell line |
| Tag : |
His |
| Protein Length : |
3510-3964 aa |
| Description : |
The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR). The amino acid 3510-3964 encodes the fourth class A motif cluster in human Megalin, termed Megalin C4. Human Megalin C4 shares 77% and 74% identity with mouse and rat Megalin C4. |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Molecular Mass : |
52 kDa |
| Endotoxin : |
< 0.10 EU/μg of the protein by the LAL method. |
| Purity : |
> 80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Shipping : |
The product is shipped at ambient temperature. |
| Reconstitution : |
Reconstitute at 250 μg/mL in PBS. |
| Reference : |
1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
2. Saito, A. et al. (1994) Proc.Natl. Aca. Sci. U. S. A. 91:9725.
3. Kantarci, S. et al. (2007) Nat. Genet 39:957. |
| N-terminal Sequence Analysis : |
Pro3510 and Thr3515 |
