Recombinant Human LRP6 protein, Mouse IgG Fc-tagged
|Product Overview :||Recombinant Human LRP6 (Ala20-Pro630) protein was fused to mouse IgG2a Fc tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin.|
|Tag :||Mouse IgG Fc|
|Predicted N Terminal :||Ala20|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 95.8 kDa. The protein migrates as 55 kDa and 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Ala20-Pro630|
|Endotoxin :||Less than 0.1 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of LRP6-552H :||
Human LRP-6 (20-630), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of LRP6-552H :||
Immobilized Mouse Dkk-1, His Tag at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag with a linear range of 10-78 ng/mL (QC tested).
|Gene Name :||LRP6|
|Official Symbol :||LRP6|
|Synonyms :||LRP6; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP-6; ADCAD2; FLJ90062; FLJ90421|
|Gene ID :||4040|
|mRNA Refseq :||NM_002336|
|Protein Refseq :||NP_002327|
|UniProt ID :||O75581|
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