Recombinant Human LYNA Protein, His/GST-tagged


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Recombinant Human LYNA Protein, His/GST-tagged

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Cat.No. : LYNA-760H
Product Overview : Human LYN, full length, amino acids M1-P512 (as in NCBI/Protein entry NP_002341.1), N- terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells.
Description : This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Source : Insect cell
Species : Human
Tag : His/GST
Bio-activity : Specific kinase activity (Pi transfer): 94 pmol/µg × min
ATP-KM: 1.4 µM
Molecular Mass : 88.47 kDa
Storage : At -80 centigrade.
For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!
Concentration : 0.222 µg/µl
Storage Buffer : 50 mM TRIS-HCl pH 8.0, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20 % glycerol
Coomassie stain :

Determination of Vmax and KM value for ATP :

Determination of KM value / Specific activity:
• Assay conditions:
60 mM HEPES-NaOH, pH 7.5
3 mM MgCl2
3 mM MnCl2
3 µM Na-orthovanadate
1.2 mM DTT
50 µg/ml PEG20.000
ATP (variable)
Substrate: Poly(Glu:Tyr)4:1, 40 µg/ml
LYN: 1 µg/ml
• Filter binding assay
MSFC membrane (Millipore)

Gene Name : LYN LYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
Official Symbol : LYN
Synonyms : LYN; LYN proto-oncogene, Src family tyrosine kinase; JTK8; p53Lyn; p56Lyn; tyrosine-protein kinase Lyn; lck/Yes-related novel protein tyrosine kinase; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; EC
Gene ID : 4067
mRNA Refseq : NM_00235
Protein Refseq : NP_002341
MIM : 165120
UniProt ID : P07948
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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