Recombinant Human LYNA Protein, His/GST-tagged LYNA-760H

Recombinant Human LYNA Protein, His/GST-tagged

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Recombinant Human LYNA Protein, His/GST-tagged

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Cat.No. : LYNA-760H
Product Overview : Human LYN, full length, amino acids M1-P512 (as in NCBI/Protein entry NP_002341.1), N- terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells.
Description : This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Source : Insect cell
Species : Human
Tag : His/GST
Bio-activity : Specific kinase activity (Pi transfer): 94 pmol/µg × min
ATP-KM: 1.4 µM
Molecular Mass : 88.47 kDa
Storage : At -80 centigrade.
For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10µl are not advisable. Avoid repeated freeze-thaw cycles!
Concentration : 0.222 µg/µl
Storage Buffer : 50 mM TRIS-HCl pH 8.0, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20 % glycerol
Coomassie stain :

Determination of Vmax and KM value for ATP :

Determination of KM value / Specific activity:
• Assay conditions:
60 mM HEPES-NaOH, pH 7.5
3 mM MgCl2
3 mM MnCl2
3 µM Na-orthovanadate
1.2 mM DTT
50 µg/ml PEG20.000
ATP (variable)
Substrate: Poly(Glu:Tyr)4:1, 40 µg/ml
LYN: 1 µg/ml
• Filter binding assay
MSFC membrane (Millipore)

Gene Name : LYN LYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
Official Symbol : LYN
Synonyms : LYN; LYN proto-oncogene, Src family tyrosine kinase; JTK8; p53Lyn; p56Lyn; tyrosine-protein kinase Lyn; lck/Yes-related novel protein tyrosine kinase; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; EC 2.7.10.2
Gene ID : 4067
mRNA Refseq : NM_00235
Protein Refseq : NP_002341
MIM : 165120
UniProt ID : P07948

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