Active Recombinant Human MAG protein, hFc-tagged

• Cat.No.: MAG-0507H
• Product Overview: Active Recombinant Human MAG protein(P20916)(Gly20-Pro516) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 20-516 aa
• Form: Supplied as 0.22μm filtered solution in PBS, 500mM Nacl (pH 7.4).
• Bio-activity: Immobilized Human Siglec-4a, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MAG myelin associated glycoprotein [ Homo sapiens ]
• Official Symbol: MAG
• Synonyms: MAG; myelin associated glycoprotein; GMA; myelin-associated glycoprotein; S MAG; sialic acid binding Ig like lectin 4A; SIGLEC 4A; SIGLEC4A; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; S-MAG; SIGLEC-4A
• Gene ID: 4099
• mRNA Refseq: NM_001199216
• Protein Refseq: NP_001186145
• MIM: 159460
• UniProt ID: P20916