| Description : |
This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
113.7 kDa |
| AA Sequence : |
MGAYARASGVCARGCLDSAGPWTMS RALRPPLPPLCFFLLLLAAAGARAG GYETCPTVQPNMLNVHLLPHTHDDV GWLKTVDQYFYGIKNDIQHAGVQYI LDSVISALLADPTRRFIYVEIAFFS RWWHQQTNATQEVVRDLVRQGRLEF ANGGWVMNDEAATHYGAIVDQMTLG LRFLEDTFGNDGRPRVAWHIDPFGH SREQASLFAQMGFDGFFFGRLDYQD KWVRMQKLEMEQVWRASTSLKPPTA DLFTGVLPNGYNPPRNLCWDVLCVD QPLVEDPRSPEYNAKELVDYFLNVA TAQGRYYRTNHTVMTMGSDFQYENA NMWFKNLDKLIRLVNAQQAKGSSVH VLYSTPACYLWELNKANLTWSVKHD DFFPYADGPHQFWTGYFSSRPALKR YERLSYNFLQVCNQLEALVGLAANV GPYGSGDSAPLNEAMAVLQHHDAVS GTSRQHVANDYARQLAAGWGPCEVL LSNALARLRGFKDHFTFCQQLNISI CPLSQTAARFQVIVYNPLGRKVNWM VRLPVSEGVFVVKDPNGRTVPSDVV IFPSSDSQAHPPELLFSASLPALGF STYSVAQVPRWKPQARAPQPIPRRS WSPALTIENEHIRATFDPDTGLLME IMNMNQQLLLPVRQTFFWYNASIGD NESDQASGAYIFRPNQQKPLPVSRW AQIHLVKTPLVQEVHQNFSAWCSQV VRLYPGQRHLELEWSVGPIPVGDTW GKEVISRFDTPLETKGRFYTDSNGR EILERRRDYRPTWKLNQTEPVAGNY YPVNTRIYITDGNMQLTVLTDRSQG GSSLRDGSLELMVHRRLLKDDGRGV SEPLMENGSGAWVRGRHLVLLDTAQ AAAAGHRLLAEQEVLAPQVVLAPGG GAAYNLGAPPRTQFSGLRRDLPPSV HLLTLASWGPEMVLLRLEHQFAVGE DSGRNLSAPVTLNLRDLFSTFTITR LQETTLVANQLREAASRLKWTTNTG PTPHQTPYQLDPANITLEPMEIRTF LASVQWKEVDGTRTRPLEQKLISEE DLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
